Splicing-independent recruitment of spliceosomal small nuclear RNPs to nascent RNA polymerase II transcripts
نویسندگان
چکیده
منابع مشابه
Splicing-independent recruitment of spliceosomal small nuclear RNPs to nascent RNA polymerase II transcripts
In amphibian oocytes, most lateral loops of the lampbrush chromosomes correspond to active transcriptional sites for RNA polymerase II. We show that newly assembled small nuclear ribonucleoprotein (RNP [snRNP]) particles, which are formed upon cytoplasmic injection of fluorescently labeled spliceosomal small nuclear RNAs (snRNAs), target the nascent transcripts of the chromosomal loops. With th...
متن کاملSplicing of Nascent RNA Coincides with Intron Exit from RNA Polymerase II
Protein-coding genes in eukaryotes are transcribed by RNA polymerase II (Pol II) and introns are removed from pre-mRNA by the spliceosome. Understanding the time lag between Pol II progression and splicing could provide mechanistic insights into the regulation of gene expression. Here, we present two single-molecule nascent RNA sequencing methods that directly determine the progress of splicing...
متن کاملBiogenesis of small nuclear RNPs.
The nucleus contains a large number of metabolically stable, 60-450-nt-long small nuclear RNAs (snRNAs) that exist in the form of ribonucleoproteins (snRNPs) (Yu et al., 1999). Each snRNP is composed of an snRNA and a set of associated RNP proteins. Based on their function and intranuclear localization, mammalian snRNPs can be classified into three major classes. The U1, U2, U4, U5 and U6 major...
متن کاملThe nuclear RNA polymerase II surveillance system targets polymerase III transcripts
A key question in nuclear RNA surveillance is how target RNAs are recognized. To address this, we identified in vivo binding sites for nuclear RNA surveillance factors, Nrd1, Nab3 and the Trf4/5–Air1/2–Mtr4 polyadenylation (TRAMP) complex poly(A) polymerase Trf4, by UV crosslinking. Hit clusters were reproducibly found over known binding sites on small nucleolar RNAs (snoRNAs), pre-mRNAs and cr...
متن کاملExplorer The nuclear RNA polymerase II surveillance system targets polymerase III transcripts
Publisher Rights Statement: This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial Share Alike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resultingwork under the same or similar license to this one. Theworkmust be attributed back to the original author and commercial use ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2007
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.200706134